[Influence of blockade of costimulation on Th1/Th2 cytokines shift in unexplained early recurrent spontaneous].

OBJECTIVE

To assess the influence of blockade of costimulation on Th1/Th2 Cytokines shift in unexplained early recurrent spontaneous abortion (URSA).

METHODS

The levels of IL-2, IFN-gamma and IL-4 were determined by ELISA in the supernatant of cultured maternal peripheral blood mononuclear cells (PBMC) from 30 patients of URSA. Cytokine synthesis was induced by activation with antigens from a choriocarcinomer cell line of JEG-3, with CTLA4Ig at concentrations of 10 microg/mL and 1 microg/mL (experiment groups), or with IgG at the same concentrations (control groups).

RESULTS

The CTLA4Ig groups showed significantly higher levels of Th2 cytokines IL-4 (P < 0.05), lower levels of Th1 cytokines IL-2 (P < 0.05) and the same levels of IFN-gamma (P > 0.05), compared with the control groups of the same concentrations respectively. The same results were found between the experiment groups of different concentration.

CONCLUSION

CTLA4Ig could switch T cell responses from Th1 to Th2 in URSA by blockade of CD80/CD86 costimulation.