Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2010
[Screen of a membrane protein molecule from Vero cells capable of binding Japanese encephalitis virus(JEV)].
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Li Yan, Shi Ying, Ding Tian-bing, Xu Zhi-kai
Аннотация
AIM: Identification of membrane protein molecules Vero cells binding JEV.
METHODS: Membrane protein extract was subject to co-immunoprecipitation (Co-IP) with JEV, and identified by mass (MS) spectrometry. The binding between specific extract protein and JEV was measured by MS, flow cytometry (FCM) and immunofluorescence assay (IFA).
RESULTS: Heat shock protein 90 beta (HSP90 beta) was identified by Co-IP and MS, and its binding JEV activity was confirmed by FCM and IFA.
CONCLUSION: HSP 90 beta from Vero cells membrane could bind JEV.
METHODS: Membrane protein extract was subject to co-immunoprecipitation (Co-IP) with JEV, and identified by mass (MS) spectrometry. The binding between specific extract protein and JEV was measured by MS, flow cytometry (FCM) and immunofluorescence assay (IFA).
RESULTS: Heat shock protein 90 beta (HSP90 beta) was identified by Co-IP and MS, and its binding JEV activity was confirmed by FCM and IFA.
CONCLUSION: HSP 90 beta from Vero cells membrane could bind JEV.