[Screen of a membrane protein molecule from Vero cells capable of binding Japanese encephalitis virus(JEV)].

AIM: Identification of membrane protein molecules Vero cells binding JEV.

METHODS

Membrane protein extract was subject to co-immunoprecipitation (Co-IP) with JEV, and identified by mass (MS) spectrometry. The binding between specific extract protein and JEV was measured by MS, flow cytometry (FCM) and immunofluorescence assay (IFA).

RESULTS

Heat shock protein 90 beta (HSP90 beta) was identified by Co-IP and MS, and its binding JEV activity was confirmed by FCM and IFA.

CONCLUSION

HSP 90 beta from Vero cells membrane could bind JEV.