Zhurnal mikrobiologii, epidemiologii i immunobiologii · 2017
PREVALENCE OF GENETIC MARKERS OF RESISTANCE TO ANTIBIOTICS IN BIOFILM-FORMING STRAINS OF OBLIGATE AND ELECTIVE ANAEROBES.
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Tsarev V N, Ippolitov E V, Nikolaeva E N
Аннотация
AIM: Comparative study of frequency of detection of genetic markers of resistance to antibiotics forming in anaerobic bacteria under the conditions of mixed biofilms in a clinical setting and comparison of data of phenotypic and genotypic methods of study.
MATERIALS AND METHODS: 66 strains of bacteria forming biofilm with PCR detection of antibiotics were studied: Streptococcus sanguinis, Streptococcus salivarius, Staphylococcus aureus, Staphylococcus epi- dermidis, Enterococcusfaecalis, Klebsiellapneumoniae, Pseudomonas aeruginosa and anaero- bic pathogens - Porphyromonasgingivalis, Tannerella forsythia, Parvinonas micra, Prevotella intermedia. Modelling of microbial biofilms in vitro and scanning electron microscopy were carried out.
RESULTS: The studied strains of resident and pathogenic microbiota were established to have genes that code resistance to P-lactam antibiotics; carbapenems, macrolides, tetra- cyclines. Genetic markers of resistance to P-lactam antibiotics (STX-M 14 MECA - cepha- losporines), including carbapenems.(VIM and NDM, but not Oxa-48), glycopeptides (VanA and VanB), macrolides (ERM), tetracycline (Tet) and QNRB plasmids (fluoroquinolones) were detected in strains by PCR.
CONCLUSION: The most frequently used preparations in dental practice - metronidazole and lincomycin (for the last 20 - 30 years) have shown the highest number of resistant strains - 52.3 and 22.7%, respectively. The frequency of detection of genetic markers of resistance to other studied preparations did not exceed 2.5 - 11.4%. Minimal quantity of resistant strains of anaerobic bacteria was detected for carbapenems and fluoroquinolones.
MATERIALS AND METHODS: 66 strains of bacteria forming biofilm with PCR detection of antibiotics were studied: Streptococcus sanguinis, Streptococcus salivarius, Staphylococcus aureus, Staphylococcus epi- dermidis, Enterococcusfaecalis, Klebsiellapneumoniae, Pseudomonas aeruginosa and anaero- bic pathogens - Porphyromonasgingivalis, Tannerella forsythia, Parvinonas micra, Prevotella intermedia. Modelling of microbial biofilms in vitro and scanning electron microscopy were carried out.
RESULTS: The studied strains of resident and pathogenic microbiota were established to have genes that code resistance to P-lactam antibiotics; carbapenems, macrolides, tetra- cyclines. Genetic markers of resistance to P-lactam antibiotics (STX-M 14 MECA - cepha- losporines), including carbapenems.(VIM and NDM, but not Oxa-48), glycopeptides (VanA and VanB), macrolides (ERM), tetracycline (Tet) and QNRB plasmids (fluoroquinolones) were detected in strains by PCR.
CONCLUSION: The most frequently used preparations in dental practice - metronidazole and lincomycin (for the last 20 - 30 years) have shown the highest number of resistant strains - 52.3 and 22.7%, respectively. The frequency of detection of genetic markers of resistance to other studied preparations did not exceed 2.5 - 11.4%. Minimal quantity of resistant strains of anaerobic bacteria was detected for carbapenems and fluoroquinolones.