Clinical nutrition ESPEN · 2022
Short Report: Choline plasma levels are related to Nrf2 transcriptional expression in chronic kidney disease?
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Mafra D, Cardozo L, Ribeiro-Alves M, Bergman P, Shiels P G, Stenvinkel P
Аннотация
BACKGROUND & AIMS: Choline is an amine osmolyte with antioxidant potential. A limited number of studies have implied that choline modulates the nuclear factor-erythroid 2-related factor 2 (Nrf 2) pathway, a major cytoprotectant system. However, there are no data regarding such an interaction in patients with chronic kidney disease (CKD). This cross-sectional pilot study therefore aimed to evaluate the possible relationship between choline plasma levels and transcriptional expression of Nrf2 in patients with CKD on hemodialysis (HD).
METHODS: This study was performed in 24 HD patients [54 ± 10 years, 14 men, BMI 26.4 ± 4.5 kg/m2]. Choline plasma levels were measured by LC-MS/MS. Nrf2 mRNA expression was measured by real-time quantitative polymerase chain reaction (rt-PCR) in isolated peripheral blood mononuclear cells (PBMC).
RESULTS: We used Pearson's correlation (rho) to determine the correlations with Nrf 2 expression and observed a positive correlation between choline plasma levels and Nrf2 (rho = 0.56, P = 0.004).
CONCLUSIONS: This finding suggests that choline may play a role in Nrf2 expression in CKD.
METHODS: This study was performed in 24 HD patients [54 ± 10 years, 14 men, BMI 26.4 ± 4.5 kg/m2]. Choline plasma levels were measured by LC-MS/MS. Nrf2 mRNA expression was measured by real-time quantitative polymerase chain reaction (rt-PCR) in isolated peripheral blood mononuclear cells (PBMC).
RESULTS: We used Pearson's correlation (rho) to determine the correlations with Nrf 2 expression and observed a positive correlation between choline plasma levels and Nrf2 (rho = 0.56, P = 0.004).
CONCLUSIONS: This finding suggests that choline may play a role in Nrf2 expression in CKD.