Медицинская библиотека · Saudi medical journal · 2011

Genotypes of hepatitis B virus in Central Anatolia, Kayseri, Turkey.

Atalay Mustafa A, Gokahmetoglu Selma, Aygen Bilgehan

СтатьяПеревод в очереди

Аннотация

OBJECTIVE

To investigate the distribution of hepatitis B virus (HBV) genotypes among patients with chronic hepatitis B in Kayseri, Turkey.

METHODS

The study took place in the Department of Microbiology, Erciyes University, Kayseri, and Iontek Laboratory, Istanbul, Turkey, from January 2005 to October 2007. One hundred and ten patients with chronic hepatitis B were included in this study. Hepatitis B virus DNA in sera were investigated by using the real-time polymerase chain reaction. Viral DNA was extracted from 200 uL of serum using the QIAamp DNA minElute kit (Qiagen, Hilden, Germany). Reaction mixture was prepared by Fluorion HBV QNP 2.0 ( Iontek, Istanbul, Turkey).

RESULTS

Genotype D was detected in 107 of 110 (97.2%) patients, however, genotyping failed in 3 patients (2.7%). No other genotypes were found.

CONCLUSION

The vast majority of Turkish patients with chronic hepatitis B have genotype D.

Для цитирования: Atalay Mustafa A, Gokahmetoglu Selma, Aygen Bilgehan. Genotypes of hepatitis B virus in Central Anatolia, Kayseri, Turkey.. Saudi medical journal. 2011.

Предметные рубрики MeSH

Adult DNA, Viral Female Genotype Hepatitis B virus Humans Male Polymerase Chain Reaction Turkey

Подготовка и перевод публикации

Обработка статьи · этапы, ошибки и управление

Загрузка состояния…

История · было → стало

Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.