Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera.

Transposon sequencing, or Tn-seq, combines transposon mutagenesis and massively parallel sequencing to allow for rapid and high-throughput identification of genes that play roles in fitness within environments of interest. The bacterial pathogen Vibrio cholerae is an excellent candidate for Tn-seq screens due to the availability of a plasmid-based in vivo transposition system and the relative ease with which the cholera disease state can be modeled in animals. This chapter will describe a method for performing Tn-seq screens on V. cholerae in the infant rabbit model of cholera.