Scandinavian journal of infectious diseases · 2011
Is sarcoidosis a rickettsiosis? An archival study.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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История · было → стало
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Svendsen Claus Bo, Milman Nils, Andersen Claus B, Rasmussen Erik M, Thomsen Vibeke Ø, Krogfelt Karen A
Аннотация
BACKGROUND: Based on earlier research, Rickettsia helvetica could possibly be involved in the pathogenesis of sarcoidosis. Rickettsiae are transmitted to humans by a tick vector, Ixodes ricinus; this tick is highly prevalent in Northern Europe. We aimed to investigate the association between evidence of rickettsiae and sarcoidosis in histological samples.
METHODS: We included formalin-fixed, paraffin-embedded mediastinal lymph node biopsies from 52 ethnic Danish patients with sarcoidosis and compared these with 50 biopsies from ethnic Danish patients with mediastinal lymphadenopathy of other causes. Samples were analysed for: (1) rickettsial DNA by real-time polymerase chain reaction (PCR) and (2) rickettsial rDNA (ribosomal DNA) by a specific fluorescence in situ hybridization technique (FISH).
RESULTS: Rickettsia was not detected in biopsies by real-time PCR and/or FISH analyses.
CONCLUSION: Our results do not support the hypothesis that Rickettsia is involved in the pathogenesis of sarcoidosis.
METHODS: We included formalin-fixed, paraffin-embedded mediastinal lymph node biopsies from 52 ethnic Danish patients with sarcoidosis and compared these with 50 biopsies from ethnic Danish patients with mediastinal lymphadenopathy of other causes. Samples were analysed for: (1) rickettsial DNA by real-time polymerase chain reaction (PCR) and (2) rickettsial rDNA (ribosomal DNA) by a specific fluorescence in situ hybridization technique (FISH).
RESULTS: Rickettsia was not detected in biopsies by real-time PCR and/or FISH analyses.
CONCLUSION: Our results do not support the hypothesis that Rickettsia is involved in the pathogenesis of sarcoidosis.