American journal of veterinary research · 1997
Application of multiplex polymerase chain reaction for rapid identification of Campylobacter jejuni and C coli associated with reproductive failure.
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Wesley I V, Sanderson T P, Larson D J, Harmon K M, Andrews J J, Miskimins D W, Zeman D H
Аннотация
OBJECTIVE: To evaluate a multiplex polymerase chain reaction (PCR) to distinguish Campylobacter jejuni from C coli as causes of reproductive failure.
PROCEDURE: Review of clinical cases of reproductive failure attributed to C jejuni or C coli.
RESULTS: A case of swine abortion was attributable to infection with C coli. The porcine abortion isolates were verified as C coli by restriction fragment length polymorphism and multiplex PCR. Cases of endometritis in a fox and in mink caused by C jejuni were reviewed, and isolates were confirmed as C jejuni by results of the multiplex PCR.
CONCLUSION: Multiplex PCR was useful in identifying C coli and C jejuni recovered from atypical cases of reproductive failure. Multiplex PCR in conjunction with conventional assays may be useful for verifying other unusual instances of campylobacteriosis.
PROCEDURE: Review of clinical cases of reproductive failure attributed to C jejuni or C coli.
RESULTS: A case of swine abortion was attributable to infection with C coli. The porcine abortion isolates were verified as C coli by restriction fragment length polymorphism and multiplex PCR. Cases of endometritis in a fox and in mink caused by C jejuni were reviewed, and isolates were confirmed as C jejuni by results of the multiplex PCR.
CONCLUSION: Multiplex PCR was useful in identifying C coli and C jejuni recovered from atypical cases of reproductive failure. Multiplex PCR in conjunction with conventional assays may be useful for verifying other unusual instances of campylobacteriosis.
Предметные рубрики MeSH
Abortion, SepticAbortion, VeterinaryAnimalsBase SequenceBlotting, SouthernCampylobacter InfectionsCampylobacter coliCampylobacter jejuniDNA, BacterialElectrophoresis, Polyacrylamide GelEndometritisFemaleFoxesMinkOligonucleotidesPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPregnancyReproductionSwineSwine Diseases