[Laboratory Diagnosis of Omsk Hemorrhagic Fever Virus and Kyasanur Forest Disease Virus].

The Omsk hemorrhagic fever virus and Kyasanur Forest disease virus, which belong to the genus Orthoflavivirus in the family Flaviviridae, are tick-borne viruses. These viruses, which were previously endemic to certain countries and regions, are now at increasing risk of spreading to new areas owing to global climate change and increased human mobility. Despite advances in real-time reverse transcription-polymerase chain reaction and serological assays, there are challenges in distinguishing these viruses from other tick-borne flavivirus infections. There is still a need for accurate and rapid point-of-care diagnostics, especially in diverse climatic conditions and medical-resource-limited settings. In this study, we described the diagnostic methods used to prevent the spread of these viruses to new regions.