Contrasting leaf structural, photosynthetic and allocation responses to elevated [CO2] in different-aged leaves of tropical fruit trees Persea americana and Annona muricata.

Responses of leaf photosynthetic traits to elevated growth [CO2] vary among species, but there is limited understanding of underlying trait trade-offs, especially for tropical species with continuous leaf formation. Persea americana and Annona muricata with significant investments in defense structures (idioblasts) were used to study the impacts of growth [CO2] (400 vs 800 μmol mol-1) on leaf structural, chemical, and photosynthetic characteristics at different leaf developmental stages. Growth at elevated [CO2] increased whole plant leaf area (ST) and whole plant average leaf dry mass per unit area (MAv) in P. americana, whereas both ST and MAv were reduced in A. muricata. Elevated [CO2] moderately reduced foliage N and P contents per dry mass in P. americana but increased in A. muricata. In P. americana, elevated [CO2] increased anthocyanin content in young leaves and decreased in mature leaves, and increased the share of idioblast tissue fraction (fI) with moderate downregulation of photosynthesis (A). In A. muricata, elevated [CO2] reduced anthocyanin content in young leaves and fI was unaffected, whereas a major downregulation in A was observed. In this species, photosynthetic downregulation was not associated with nutrient starvation, but occurred due to direct inhibition of stomatal conductance by elevated [CO2], ultimately limiting leaf development and growth and curbing Vcmax and Jmax in mature leaves. These results demonstrate a limited impact of primary/secondary metabolism trade-off on photosynthetic response to growth [CO2], underscore major species differences in response to elevated [CO2] and emphasize the impact of leaf age in determining whole plant growth response.