Viruses · 2023
Novel Amplicon-Based Sequencing Approach to West Nile Virus.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Diagne Moussa Moïse, Ndione Marie Henriette Dior, Mencattelli Giulia, Diallo Amadou, Ndiaye El Hadji, Di Domenico Marco, Diallo Diawo, Kane Mouhamed, Curini Valentina, Top Ndeye Marieme, Marcacci Maurilia, Mbanne Maïmouna, Ancora Massimo, Secondini Barbara, Di Lollo Valeria, Teodori Liana, Leone Alessandra, Puglia Ilaria, Gaye Alioune, Sall Amadou Alpha, Loucoubar Cheikh, Rosà Roberto, Diallo Mawlouth, Monaco Federica, Faye Ousmane, Cammà Cesare, Rizzoli Annapaola, Savini Giovanni, Faye Oumar
Аннотация
West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world. It is therefore crucial to properly control the dispersion of these lineages, especially because some have a greater health impact on public health than others. This work describes the development and validation of a novel whole-genome amplicon-based sequencing approach to West Nile virus. This study was carried out on different strains from lineage 1 and 2 from Senegal and Italy. The presented protocol/approach showed good coverage using samples derived from several vertebrate hosts and may be valuable for West Nile genomic surveillance.