Bioresource technology · 2011
Investigation of enzyme formulation on pretreated switchgrass.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Falls Matthew, Shi Jian, Ebrik Mirvat A, Redmond Tim, Yang Bin, Wyman Charles E, Garlock Rebecca, Balan Venkatesh, Dale Bruce E, Pallapolu V Ramesh, Lee Y Y, Kim Youngmi, Mosier Nathan S, Ladisch Michael R, Hames Bonnie, Thomas Steve, Donohoe Bryon S, Vinzant Todd B, Elander Richard T, Warner Ryan E, Sierra-Ramirez Rocio, Holtzapple Mark T
Аннотация
This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime+ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO(2)). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0-2%) for the pretreatments with low xylan content (dilute-acid, SO(2)). Xylanase addition also improved overall yields with lime+ball-milling and SO(2) achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime+ball-milling obtained an enzymatic yield of 92.3kg of sugar digested/kg of protein loaded.