Solvent effect on the determination of sulfamethazine by room-temperature photochemically induced fluorescence.

Room-temperature photochemically-induced fluorescence (RTPF) was applied to the determination of sulfamethazine (SMT) in methanol, ethanol and 2-propanol. Optimal ultraviolet irradiation times ranged between 2 and 6 min. Linear calibration graphs were obtained over a concentration range of more than one order of magnitude. The relative standard deviations were within the range 1.4-2.2%. Limits of detection were between 40 and 80 ng/ml. The method was evaluated for its applicability to the analysis of SMT in pharmaceutical formulations.