Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2022
[Analysis of material preparation, anti-bacterial activity of Porphyromonas gingivalis and cell bioassays of TiO2-PEEK-CRGDS].
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Xu Xin-Yi, Zhang Ying-Lian
Аннотация
PURPOSE: To modify polyetheretherketone(PEEK) composite material with nanometer titanium dioxide (TiO2) and CRGDS peptide to prepare TiO2-PEEK-CRGDS, and evaluate its anti- activity of Porphyromonas gingivalis and effect on cell activity.
METHODS: TiO2-PEEK was prepared by plasma immersion ion implantation(PⅢ), then immersed into gelatin solution of pentapeptides of CRGDS, and freeze-dried. The same diameter and thickness of PEEK was set as control. Scanning electron microscope (SEM) was used to observe the three-dimensional topography and roughness on the surface of the specimen, colony count was calculated to evaluate the survival of Porphyromonas gingivalis, living/dead cell count was used to detect the activity of human gingival fibroblasts(HGFs), CCK-8 assay was used to detect the proliferation ability of HGFs. The quantitative data was indicated as and analyzed by using GraphPad Prism 7.0 software package.
RESULTS: The antibacterial rates of PEEK, TiO2-PEEK and TiO2-PEEK-CRGDS were 40.3%, 57.2% and 60.3%, respectively. At 12 days, the ability of TiO2-PEEK-CRGDS to promote the growth of human gingival fibroblasts was significantly different from that of the TiO2-PEEK and PEEK group(P＜0.05).
CONCLUSIONS: The optimized TiO2-PEEK-CRGDS has better hydrophilicity than pure titanium and PEEK, and can significantly promote the proliferation of gingival fibroblasts.
METHODS: TiO2-PEEK was prepared by plasma immersion ion implantation(PⅢ), then immersed into gelatin solution of pentapeptides of CRGDS, and freeze-dried. The same diameter and thickness of PEEK was set as control. Scanning electron microscope (SEM) was used to observe the three-dimensional topography and roughness on the surface of the specimen, colony count was calculated to evaluate the survival of Porphyromonas gingivalis, living/dead cell count was used to detect the activity of human gingival fibroblasts(HGFs), CCK-8 assay was used to detect the proliferation ability of HGFs. The quantitative data was indicated as and analyzed by using GraphPad Prism 7.0 software package.
RESULTS: The antibacterial rates of PEEK, TiO2-PEEK and TiO2-PEEK-CRGDS were 40.3%, 57.2% and 60.3%, respectively. At 12 days, the ability of TiO2-PEEK-CRGDS to promote the growth of human gingival fibroblasts was significantly different from that of the TiO2-PEEK and PEEK group(P＜0.05).
CONCLUSIONS: The optimized TiO2-PEEK-CRGDS has better hydrophilicity than pure titanium and PEEK, and can significantly promote the proliferation of gingival fibroblasts.