[Analysis of material preparation, anti-bacterial activity of Porphyromonas gingivalis and cell bioassays of TiO2-PEEK-CRGDS].

PURPOSE: To modify polyetheretherketone(PEEK) composite material with nanometer titanium dioxide (TiO2) and CRGDS peptide to prepare TiO2-PEEK-CRGDS, and evaluate its anti- activity of Porphyromonas gingivalis and effect on cell activity.

METHODS

TiO2-PEEK was prepared by plasma immersion ion implantation(PⅢ), then immersed into gelatin solution of pentapeptides of CRGDS, and freeze-dried. The same diameter and thickness of PEEK was set as control. Scanning electron microscope (SEM) was used to observe the three-dimensional topography and roughness on the surface of the specimen, colony count was calculated to evaluate the survival of Porphyromonas gingivalis, living/dead cell count was used to detect the activity of human gingival fibroblasts(HGFs), CCK-8 assay was used to detect the proliferation ability of HGFs. The quantitative data was indicated as and analyzed by using GraphPad Prism 7.0 software package.

RESULTS

The antibacterial rates of PEEK, TiO2-PEEK and TiO2-PEEK-CRGDS were 40.3%, 57.2% and 60.3%, respectively. At 12 days, the ability of TiO2-PEEK-CRGDS to promote the growth of human gingival fibroblasts was significantly different from that of the TiO2-PEEK and PEEK group(P＜0.05).

CONCLUSIONS

The optimized TiO2-PEEK-CRGDS has better hydrophilicity than pure titanium and PEEK, and can significantly promote the proliferation of gingival fibroblasts.