Cell stem cell · 2018
Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Wang Mei, Liu Xixi, Chang Gang, Chen Yidong, An Geng, Yan Liying, Gao Shuai, Xu Yanwen, Cui Yueli, Dong Ji, Chen Yuhan, Fan Xiaoying, Hu Yuqiong, Song Ke, Zhu Xiaohui, Gao Yun, Yao Zhaokai, Bian Shuhui, Hou Yu, Lu Jiahao, Wang Rui, Fan Yong, Lian Ying, Tang Wenhao, Wang Yapeng, Liu Jianqiao, Zhao Lianming, Wang Luyu, Liu Zhaoting, Yuan Renpei, Shi Yujia, Hu Boqiang, Ren Xiulian, Tang Fuchou, Zhao Xiao-Yang, Qiao Jie
Аннотация
Spermatogenesis generates mature male gametes and is critical for the proper transmission of genetic information between generations. However, the developmental landscapes of human spermatogenesis remain unknown. Here, we performed single-cell RNA sequencing (scRNA-seq) analysis for 2,854 testicular cells from donors with normal spermatogenesis and 174 testicular cells from one nonobstructive azoospermia (NOA) donor. A hierarchical model was established, which was characterized by the sequential and stepwise development of three spermatogonia subtypes, seven spermatocyte subtypes, and four spermatid subtypes. Further analysis identified several stage-specific marker genes of human germ cells, such as HMGA1, PIWIL4, TEX29, SCML1, and CCDC112. Moreover, we identified altered gene expression patterns in the testicular somatic cells of one NOA patient via scRNA-seq analysis, paving the way for further diagnosis of male infertility. Our work allows for the reconstruction of transcriptional programs inherent to sequential cell fate transition during human spermatogenesis and has implications for deciphering male-related reproductive disorders.