[Expression of lethal gene mRNA on placenta villi in patients with spontaneous abortion].

OBJECTIVE

To study the role of lethal gene: including retinoic X receptor (RXR)alpha, N-myc and transcript enhancer factor (TEF)-1 in human spontaneous abortion.

METHODS

The levels of RXRalpha, N-myc and TEF-1 mRNA expression on placenta villi from 38 spontaneous abortion women and 33 normal early pregnant women were examined by reverse transcription, polymerase chain reaction.

RESULTS

(1) The levels of RXRalpha mRNA on placenta villi from the abortion group were significantly decreased as compared with those from normal group (0.4 +/- 0.3 versus 0.6 +/- 0.3, P < 0.05); There were no significant differences in the levels of N-myc mRNA expression between the abortion group and normal group (2.1 +/- 1.2 versus 2.2 +/- 0.9, P > 0.05). The levels of TEF-1 mRNA on placenta from abortion group were significantly lower than those from normal group (1.6 +/- 1.1, 2.3 +/- 1.2, P < 0.05); (2) The levels of RXR alpha mRNA, TEF-1 mRNA in recurrent abortion group were significantly lower than those from non-recurrent group (0.3 +/- 0.2 versus 0.6 +/- 0.4, P < 0.05, 1.0 +/- 1.1 versus 1.9 +/- 1.2, P < 0.05).

CONCLUSION

It was suggested that the lower expression of RXR alpha mRNA, TEF-1 mRNA may play an important role in the pathogenesis of spontaneous abortion especially in recurrent abortion.