Optimization of Cell Culture for Human Adenovirus Isolation and Propagation.

OBJECTIVES

This study aimed to establish the optimal cell culture conditions for the isolation and characterization of human adenovirus (HAdV), a causative agent of respiratory infections.

METHODS

HAdV types 3, 5, and 7 were used to determine the optimal culture conditions of HAdV in three cell lines: HeLa, A549, and Vero. Based on these optimized conditions, virus isolation was attempted using 66 HAdV-positive clinical specimens of various serotypes collected by the Korea Respiratory viruses Integrated Surveillance System.

RESULTS

Cell culture experiments for HAdV showed that the HeLa cells incubated at 34℃ supported the highest level of viral replication, as confirmed by real-time PCR (polymerase chain reaction). Using these optimized conditions, 11 HAdV types were isolated from clinical specimens. Of the 66 specimens tested, 65 showed successful viral growth with an isolation rate of 98.5%.

CONCLUSIONS

This study successfully established optimized cell culture conditions for isolating HAdV strains. The Korea Disease Control and Prevention Agency will continue to secure biological resources by isolating and culturing various pathogens to support further characterization and follow-up research.