Zhurnal mikrobiologii, epidemiologii i immunobiologii · 2015
[Detection of West Nile virus RNA and specific antibodies in blood donors of Volgograd region].
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Karan L S, Fedorova M V, Gridneva K A, Chaika A N, Romasova E I
Аннотация
AIM: Study infection level by West Nile virus (WNV) of donors living on the territory of West Nile fever (WNF) foci with long-term activity, and evaluate the possibility of non-transmissible transfer of the causative agent during use of donor blood.
MATERIALS AND METHODS: Blood sera (432) collected in August and September 2012 in Volgograd and Volzhsky cities were studied by PCR and EIA.
RESULTS: Antibodies to WNV were detected in 16.2% (70/432); 8.8% (38/432) donors had IgG; 0.93% (4/432)--IgM and 6.5% (28/432)--IgG and IgM antibodies simultaneously. WNV RNA was detected in one of the samples in the last group. Thus, 5 donors (1.1%, 5/432) were at the early stages after infection.
CONCLUSION: The data obtained give evidence on the necessity to execute control of donor blood by PCR and EIA in WNF foci with long-term activity.
MATERIALS AND METHODS: Blood sera (432) collected in August and September 2012 in Volgograd and Volzhsky cities were studied by PCR and EIA.
RESULTS: Antibodies to WNV were detected in 16.2% (70/432); 8.8% (38/432) donors had IgG; 0.93% (4/432)--IgM and 6.5% (28/432)--IgG and IgM antibodies simultaneously. WNV RNA was detected in one of the samples in the last group. Thus, 5 donors (1.1%, 5/432) were at the early stages after infection.
CONCLUSION: The data obtained give evidence on the necessity to execute control of donor blood by PCR and EIA in WNF foci with long-term activity.