Veterinary surgery : VS · 2008
TP53 gene mutations in canine osteosarcoma.
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Kirpensteijn Jolle, Kik Marja, Teske Erik, Rutteman Gerard R
Аннотация
OBJECTIVE: To investigate mutations of the TP53 gene in canine osteosarcoma (OS).
STUDY DESIGN: Clinical historic cohort study.
ANIMALS: Client-owned dogs.
METHODS: OS (n=59) were screened for mutations of the complete TP53 gene using polymerase chain reaction and the mutation was analyzed by single-strand conformational polymorphism. Clinical outcome of dogs with TP53-mutated OS were compared with dogs with OS without a mutation after complete surgical excision of the primary tumor.
RESULTS: TP53 gene mutations were observed in 24 of 59 (40.7%) OS; 3 mutated OS had 2 mutations. The alterations consisted mainly of point mutations (74%). Dogs with mutated OS had a significantly shorter survival time (ST) after surgery than dogs with normal tumor TP53 gene expression (P=.03). Other significant prognosticators for ST and disease-free interval included elevated serum alkaline phosphatase (P<.01) and tumor grade (P=.01).
CONCLUSION: TP53 genetic mutations are common in canine OS and may have a prognostic value.
CLINICAL RELEVANCE: Mutations of the TP53 gene may influence survival and should be considered when evaluating canine OS.
STUDY DESIGN: Clinical historic cohort study.
ANIMALS: Client-owned dogs.
METHODS: OS (n=59) were screened for mutations of the complete TP53 gene using polymerase chain reaction and the mutation was analyzed by single-strand conformational polymorphism. Clinical outcome of dogs with TP53-mutated OS were compared with dogs with OS without a mutation after complete surgical excision of the primary tumor.
RESULTS: TP53 gene mutations were observed in 24 of 59 (40.7%) OS; 3 mutated OS had 2 mutations. The alterations consisted mainly of point mutations (74%). Dogs with mutated OS had a significantly shorter survival time (ST) after surgery than dogs with normal tumor TP53 gene expression (P=.03). Other significant prognosticators for ST and disease-free interval included elevated serum alkaline phosphatase (P<.01) and tumor grade (P=.01).
CONCLUSION: TP53 genetic mutations are common in canine OS and may have a prognostic value.
CLINICAL RELEVANCE: Mutations of the TP53 gene may influence survival and should be considered when evaluating canine OS.