Studies of chemical and biological properties of 99mTc-DMS (dimercaptosuccinic acid)--renal imaging agent.

Studies of the chemistry of preparation of tin chelate 2.3-dimercaptosuccinic acid (DMS) complexed with 99mTc with respect to its biological properties have been made. We have varied molar ratio of reactants (DMS:Sn/II/), concentration of Sn-DMS chelate and pH of 99mTc-DMS injection solution and examined their influence on biodistribution. Biodynamics of labeled solution at time intervals of from 0.5 to 4 h have also been measured. Animal experiments were carried out on mice and rats. Radiochemical purity was checked by paper chromatography, thin-layer chromatography, and gel-filtration through Bio-Gel P-2. A sterile, pyrogen-free lyophilized "kit" for labeling, stable for 6 months, was made on the basis of achieved results. The results of its clinical application are also reported.