Fertility and sterility · 2001
Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique.
Клиническое исследованиеСтатьяМногоцентровое исследованиеНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Mukaida T, Nakamura S, Tomiyama T, Wada S, Kasai M, Takahashi K
Аннотация
OBJECTIVE: Clinical application of vitrification for the cryopreservation of human blastocysts.
DESIGN: Clinical trial of vitrification of human blastocysts.
SETTING: Private assisted reproductive technology clinic.
PATIENT(S): Supernumerary blastocysts after fresh blastocyst transfer were vitrified for subsequent transfer.
INTERVENTION(S): Culture of pronuclear embryos to the blastocyst stage in sequential media and subsequent vitrification of supernumerary blastocysts using a cryoloop technique.
MAIN OUTCOME MEASURE(S): Clinical outcome after transfer of vitrified blastocysts.
RESULT(S): A total of 60 vitrified blastocysts from 21 patients were warmed, and the survival rate at 2 hours after warming was 63%. Six clinical pregnancies were achieved after 19 transfers. One healthy baby was born, four pregnancies are ongoing, and one ended in miscarriage.
CONCLUSION(S): Human blastocysts can be successfully vitrified by suspension on a small nylon loop and a direct plunge into liquid nitrogen. A delivery and ongoing pregnancies prove the safety of this method. This report documents the first successful pregnancy and delivery achieved by blastocyst vitrification using the cryoloop containerless technique.
DESIGN: Clinical trial of vitrification of human blastocysts.
SETTING: Private assisted reproductive technology clinic.
PATIENT(S): Supernumerary blastocysts after fresh blastocyst transfer were vitrified for subsequent transfer.
INTERVENTION(S): Culture of pronuclear embryos to the blastocyst stage in sequential media and subsequent vitrification of supernumerary blastocysts using a cryoloop technique.
MAIN OUTCOME MEASURE(S): Clinical outcome after transfer of vitrified blastocysts.
RESULT(S): A total of 60 vitrified blastocysts from 21 patients were warmed, and the survival rate at 2 hours after warming was 63%. Six clinical pregnancies were achieved after 19 transfers. One healthy baby was born, four pregnancies are ongoing, and one ended in miscarriage.
CONCLUSION(S): Human blastocysts can be successfully vitrified by suspension on a small nylon loop and a direct plunge into liquid nitrogen. A delivery and ongoing pregnancies prove the safety of this method. This report documents the first successful pregnancy and delivery achieved by blastocyst vitrification using the cryoloop containerless technique.