Biologicals : journal of the International Association of Biological Standardization · 2017
Microbial expression of Exendin-4 analog and its efficacy in mice model.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Kodaganti Bhargav Prasad, Mukunda Pavithra, Dakshinamurthy Pravinkumar, Manjunath Yogendra, Shenoy Bharath Ravindra, Kamanagowda Veeresha, Natarajan Bairavabalakumar, Maliwalave Amol, Unnikrishnan Divya, Murugesan Sathyabalan, Halan Vivek, Ghosh Maloy, Maity Sunit
Аннотация
Exendin-4 is a GLP 1 agonist incretin-mimetic peptide hormone comprising 39 amino acids. Exenatide (Byetta®) is a chemically synthesized version of Exendin-4 with an additional C-terminal amidation. Exenatide acts as a GLP-1 receptor agonist. This paper illustrates the method adopted for cloning, fermentation and purification of recombinant Exendin-4 analog expressed in Escherichia coli. The biologically expressed analog was extensively characterized using different orthogonal methods to confirm their biological activity and physicochemical properties. It was observed that the expressed analog showed comparable functional properties as that of Byetta® irrespective of their modes of development. Further, in vivo efficacy of the recombinant Exendin-4 analog was studied in Oral Glucose Tolerance Test (OGTT) in mice models. Byetta® and Exendin-4 analog treated groups showed comparable glucose lowering activity in the OGTT model.