Microbial induce carbonate precipitation derive bio-concrete formation: A sustainable solution for carbon sequestration and eco-friendly construction.

The microbial-induced calcium carbonate precipitation (MICP) technique has high potential in the development of bio-concrete, enhancing the strength, durability, and self-healing properties of construction materials. In this review work, we have explored the crucial role of microorganisms in carbon sequestration, microbial methods in CaCO3 synthesis, and the application of bio-concrete formation, based on the SCOPUS database from 2010 to 2024. The production of construction materials consumes a significant amount of energy, which can emit high amounts of carbon dioxide (CO2) into the atmosphere. As a sustainable solution, researchers are working to introduce novel construction biomaterials through MICP, which play a key role in CO2 sequestration to address this issue. Herein, microorganisms (bacteria) can utilize CO2 through multiple absorption processes, converting it into value-added compounds or inducing CaCO3 precipitation. For example, specific bacteria like Bacillus cereus, Bacillus sphaericus, Bacillus pasteurii, Bacillus subtilis, and Bacillus megatherium are known for their capability to thrive in alkaline conditions and play a key role in bio-concrete formation. Furthermore, it has been highlighted that the bio-concrete ability to sequester CO2 through the carbonation process, emphasizes the roles of urease activity and carbonic anhydrase (CA) in bio-concrete. Overall, this paper provides a complete synopsis of recent research on the formation of bio-concrete through MICP and the various elements influencing the technique, including cementation solution, temperature, injection, pH, and bacteria. This suggests that emerging trends in bio-concrete utilization could significantly reduce CO2 emissions while enhancing the strength of non-reinforced concrete.