Novel fabrication and biological characterizations of AgNPs-decorated PEEK with gelatin functional nanocomposite to improve superior biomedical applications.

There is an increasing interest in the use of polyether ether ketone (PEEK) for biomedical applications. Herein, we have developed silver nanoparticles (AgNPs) decorated PEEK with gelatin (GEL) nanocomposite hydrogel with enhanced antibacterial and biocompatibility through the blending method. The prepared highly porous PEEK/GEL/AgNPs nanocomposite hydrogel was characterized using SEM, TEM, FTIR, and XRD analysis. The SEM image showed that AgNPs were encapsulated in the porous PEEK/GEL hydrogel; within this porous hydrogel, the AgNPs were homogeneously dispersed. Furthermore, the tensile strength, flexural strength, and Young's modulus were 99 ± 2.4 MPa, 154 ± 7.7 MPa, and 2.3 ± 0.1 GPa, respectively, when AgNPs were added to PEEK/GEL hydrogels it exhibited the mechanical performances. The antibacterial assays demonstrate that the AgNPs-decorated PEEK/GEL nanocomposite hydrogel effectively inhibits the antibacterial effect against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria, respectively. Then, MC3T3-E1 cells demonstrated that the AgNPs-decorated PEEK/GEL nanocomposite hydrogel significantly enhanced cell viability and superior alkaline phosphatase (ALP) activity compared with PEEK/GEL hydrogel. This work opens a new avenue of the facile and effective modification of PEEK/GEL/AgNPs nanocomposite hydrogel has increased in vitro antibacterial and biocompatibility properties has great potential to be used as biomedical applications.