Potential of ethyl acetate and ethanol extracts of Bintaro (Cerbera manghas L.) seeds as bioinsecticides against Aedes aegypti (Diptera: Culicidae).

Research on vector control with bioinsecticides is still being done to reduce the negative impacts of employing pesticides. Reduced insecticide use should prevent mosquitoes from harming the environment and from developing resistance to the active components in insecticides. Botanical insecticides are derived from plants that have bioactive compounds (toxic to insects) that readily break down in the environment, do not pollute the ecosystem, and are more selective and safer than conventional pesticides. The point of this study is to find out what happens to Ae. aegypti when ethyl acetate and ethanol are mixed with a 96% solution of Bintaro fruit seeds. Samples of adult mosquitoes aged 3-5 days were 1120 with three replicates. The research design is experimental, with the post-test only being a controlled group design. Recording Mosquito mortality was recorded after 24 h of observation, while the effects of adult mosquito toxicity sprays were observed for 20 min. Giving mosquitoes ethyl acetate and 96% ethanol extract solutions of C. manghas fruit seeds killed a lot of them. It was found that the ethyl acetate solutions had LC50 values of 22,594 (225,940 ppm) and LC90 values of 92,242 (922,420 ppm). The values for the 96% ethanol extract solutions were 5,207 (52,070 ppm) and 10,436 (104,360 ppm) in 100 ml of distilled water. A one-way ANOVA test was done on the n-hexane and 96% ethanol extracts of C. manghas seeds and found that the two solutions were quite different. There was a 0.001 (p < 0.05) difference between the yields of the maceration method with 96% ethanol and the yields of ethyl acetate for each concentration of Bintaro seed extract.