High-temperature ultrafast ChipHPLC-MS.

Herein, we present a miniaturized chip-based HPLC approach coupled to electrospray ionization mass spectrometry utilizing temperature to achieve high-speed separations. The approach benefits from the low thermal mass of the microfluidic chip and can form an electrospray from the pre-heated mobile phase. With the help of this technology, isothermal and temperature-programmable operations up to 130°C were pursued to perform reversed-phase separations of pesticides in methanol and ethanol-containing eluents in less than 20 s.