Cell · 2020
Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Cao Yunlong, Su Bin, Guo Xianghua, Sun Wenjie, Deng Yongqiang, Bao Linlin, Zhu Qinyu, Zhang Xu, Zheng Yinghui, Geng Chenyang, Chai Xiaoran, He Runsheng, Li Xiaofeng, Lv Qi, Zhu Hua, Deng Wei, Xu Yanfeng, Wang Yanjun, Qiao Luxin, Tan Yafang, Song Liyang, Wang Guopeng, Du Xiaoxia, Gao Ning, Liu Jiangning, Xiao Junyu, Su Xiao-Dong, Du Zongmin, Feng Yingmei, Qin Chuan, Qin Chengfeng, Jin Ronghua, Xie X Sunney
Аннотация
The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 convalescent patients. From 8,558 antigen-binding IgG1+ clonotypes, 14 potent neutralizing antibodies were identified, with the most potent one, BD-368-2, exhibiting an IC50 of 1.2 and 15 ng/mL against pseudotyped and authentic SARS-CoV-2, respectively. BD-368-2 also displayed strong therapeutic and prophylactic efficacy in SARS-CoV-2-infected hACE2-transgenic mice. Additionally, the 3.8 Å cryo-EM structure of a neutralizing antibody in complex with the spike-ectodomain trimer revealed the antibody's epitope overlaps with the ACE2 binding site. Moreover, we demonstrated that SARS-CoV-2-neutralizing antibodies could be directly selected based on similarities of their predicted CDR3H structures to those of SARS-CoV-neutralizing antibodies. Altogether, we showed that human neutralizing antibodies could be efficiently discovered by high-throughput single B cell sequencing in response to pandemic infectious diseases.