[GIS: CAPABILITIES OF DATA ANALYSIS OF PHENO- AND GENOTYPING Of EL TOR 01 SEROGROUP CHOLERA VIBRIOS ISOLATED FROM AQUATIC OBJECTS OF THE ENVIRONMENT IN RUSSIA FEDERATION].

AIM: Application of the authors' GIS <<Cholera 1989-2014>> for systematization of atoxigenic strains ofserogroup 01 choleravibrios (ctxAB-tcpA-, ctxAB-tcpA+), isolated from aquatic objects of the environment by pheno- and genotype.

MATERIALS AND

METHODS

A sample of 304 Vibrio cholerae 01 strains was studied. Isolation of 39 genes related to pathogenicity was carried out Discrimination ability of a set of genes was determined by Simpson formula. Cluster analysis was carried out by UPGMA method.

RESULTS

Analysis of multi-year data on aquatic V cholerae Ο1 strains in country's subject was carried out using GIS. Apossibility of systematization ofphenotypes of the isolated strains by defined parameters was shown. An experimental program for detectior of presence/lack ofvarious genes and their combinations for genotyping was developed. Conclusion GIS was established to allow to carry out analysis of phenotypes by defined parameters, as well as implement approximate systematization of genotypes of atoxigenic strains of cholera vibrios Ο1 by optimally sufficient detection of 14 genes.