[Use of peroxidase-labeled antigen of tick-borne encephalitis virus for analysis of specific immunoglobulin response and the evaluation of its diagnostic usefulness for detecting the etiological factor of human neurologic infections].

Peroxidase labelled virus of tick-borne encephalitis (k.z.m.) was used for humoral immune response analysis in humans. Dot-ELISA methods elaborated in a version of "catching antibodies" (cAb-ELISA) was compared in respect to its diagnostic usefulness to indirect immunoenzymatic method (i-ELISA) and to haemagglutination inhibition test (OZHA). High sensitivity and diagnostic value of both methods were confirmed. As compared to i-ELISA, cAb-ELISA was characterised by higher specificity of IgM antibody determination resulting from a lack of influence of rheumatoid factor and specific IgG on above mentioned determinations of antiviral IgM in cAb-ELISA. Moreover, parallel analysis by cAb-ELISA technique of serum and cerebro-spinal fluid permits to evaluate in a simple manner a synthesis of specific viral antibodies present in central nervous system.