Application of Response Surface Methodology (RSM) in the statistical evaluation of biodiesel production from the neutral lipids of the Coelastrella-Nannochloropsis consortium.

The paramount challenge in economically workable microalgal biodiesel production is the selection of a competent catalyst to improve the fatty acid methyl ester yield with desirable fatty acid composition. Though countless researchers have explored different homogeneous and heterogeneous catalysts to improve the transesterification efficacy, achieving greater biodiesel production from the neutral lipids of the microalgal consortium using a statistical tool, response surface methodology is scarce. Thus, the present study applied Response surface methodology to statistically analyze the biodiesel production from the neutral lipids of the indigenous Coelastrella-Nannochloropsis consortium (CNC) on the way to commercial feasibility. Onset of the study, the neutral lipids and acid value of the CNC were determined to be 18.74% and 2.73%, respectively. The transesterification of the neutral lipids of CNC was optimized through the coded factors in the RSM for various reaction parameters as combined influence viz., (i) Catalyst dose: methanol volume, (ii) Catalyst dose: reaction time; (iii) Catalyst dose: reaction temperature, (iv) Time: temperature, (v) time: methanol volume, (vi) temperature: methanol volume. Based on the ANOVA, coefficient determination, 2% KOH, 2 h time, 70 °C temperature, and 9 mL methanol volume were ascertained to be optimal values to accomplish 92% biodiesel production. Further, the biodiesel has desirable palmitic, palmitoleic, stearic, oleic, linoleic, and linolenic acids, with palmitic acid as the prevalent fatty acid contributing 16-18%. In addition, the tested fuel properties of CNC biodiesel satisfy international biodiesel standards.