International urogynecology journal and pelvic floor dysfunction · 2009
Genome-based expression profiles as a single standardized microarray platform for the diagnosis of experimental interstitial cystitis: an array of 75 genes model.
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Tseng Ling-Hong, Chen Ilene, Chen Ming-Yang, Lee Chyi-Long, Lo Tsia-Shu, Lloyd L Keith
Аннотация
INTRODUCTION AND HYPOTHESIS: To investigate the molecular signature underlying experimental interstitial cystitis (IC) using cDNA microarray.
METHODS: Microarray gene expression profiles are studied in bladder epithelium of C57BL/6 mice with ovalbumin or substance P-induced experimental IC versus Escherichia coli lipopolysaccharide-induced bacterial cystitis.
RESULTS: Main findings are summarized as follows: firstly, a "75-gene" model was discovered to contain high expressions of bladder epithelium which feature in experimental IC. Secondly, glucose, lipid, nucleotide, xenobiotics, and amino acid metabolisms are involved in. Thirdly, T-cell-mediated immune and inflammatory responses are observed. Fourthly, Wnt, Tgf-beta, Mapk, and insulin growth factor receptor signaling pathways are also involved in. In addition, experimental IC leads to Ephrin- and Semaphorin-mediated axon guidance promoting parasympathetic inflammatory reflexes.
CONCLUSIONS: Further characterization of human IC-induced gene expression profiles would enable the use of genome-based expression profiling for the therapeutic targets and diagnosis of IC.
METHODS: Microarray gene expression profiles are studied in bladder epithelium of C57BL/6 mice with ovalbumin or substance P-induced experimental IC versus Escherichia coli lipopolysaccharide-induced bacterial cystitis.
RESULTS: Main findings are summarized as follows: firstly, a "75-gene" model was discovered to contain high expressions of bladder epithelium which feature in experimental IC. Secondly, glucose, lipid, nucleotide, xenobiotics, and amino acid metabolisms are involved in. Thirdly, T-cell-mediated immune and inflammatory responses are observed. Fourthly, Wnt, Tgf-beta, Mapk, and insulin growth factor receptor signaling pathways are also involved in. In addition, experimental IC leads to Ephrin- and Semaphorin-mediated axon guidance promoting parasympathetic inflammatory reflexes.
CONCLUSIONS: Further characterization of human IC-induced gene expression profiles would enable the use of genome-based expression profiling for the therapeutic targets and diagnosis of IC.
Предметные рубрики MeSH
AnimalsCluster AnalysisCystitis, InterstitialEphrinsEscherichia coliGene Expression ProfilingLipopolysaccharidesLymphocyte ActivationMAP Kinase Signaling SystemMiceMice, Inbred C57BLNitric Oxide Synthase Type IIIOligonucleotide Array Sequence AnalysisOvalbuminPAX2 Transcription FactorSemaphorinsSubstance PTranscriptomeUp-RegulationWnt Signaling Pathway