[Effect of actinomycin D, cycloheximide and hypertonic concentrations of NaCl on the reproduction of the tick-borne encephalitis virus].

Synthesis of virus-specific polypeptides occurs in pig embryo kidney cells infected with tick-borne encephalitis virus and treated with actinomycin D, cycloheximide, and hypertonic concentrations of NaCl in various combinations. Despite the fact that virus-specific matrix RNAs function under such conditions, production of infectious virus particles is inhibited considerably. Virus yield is greatly decreased by treatment with actinomycin D alone and by combined effect of actinomycin D and cycloheximide. Sodium chloride and combined treatment of the infected cells with cycloheximide and NaCl do not affect the intact virus production. The mechanism of the inhibiting effect of actinomycin D on production of infectious tick-borne encephalitis virus is assumed to consist in the impairment of the synthesis of virus-specific ribonucleic acid and/or in marked reduction of its infectivity. Actinomycin D may possibly inhibit somehow the morphogenesis of intact virions of tick-borne encephalitis virus.