Formaldehyde inhibits proliferation of bronchial epithelial cells by down-regulating miR-375.

Objective

To study the effect of formaldehyde on the proliferation of human bronchial epithelial cells 16HBE and to explore its mechanism.

Methods

MTT assay was used to detect the inhibition rate of formaldehyde-treated 16HBE cells; FCOH + miR-375 group (transfected miR-375 mimics), FCOH + miR-con group (transfected miR-con), FCOH + si-KLF4 group (transfected si-KLF4) and FCOH + si-con group (transfected si-con), were transfected into 16HBE cells by liposome method, then treated with formaldehyde 200 μmol/L for 24 h; qRT-PCR was used to detect the expression of miR-375 in each group; the protein expression of KLF4 in each group was detected by Western blot. The fluorescence activity of each group was detected by dual-fluorescein gene detection assay.

Results

Compared with 16HBE cells in Control group, the expression of miR-375 was significantly decreased in FCOH group, cell proliferation was significantly decreased, and KLF4 expression was significantly increased (p < .05). Overexpression of miR-375 and KLF4 knockdown could reverse the inhibition effect of formaldehyde on proliferation of 16HBE cells; KLF4 is a target of miR-375. KLF4 could reverse the promotion of miR-375 on the proliferation of formaldehyde-treated 16HBE cells.

Conclusion

Formaldehyde can inhibit the proliferation of human bronchial epithelial cells. The mechanism may be related to the down-regulation of miR-375 targeting KLF4, which will provide support for the treatment of chronic respiratory diseases.