High-pressure liquid chromatographic determination of thioridazine and its major metabolites in biological tissues and fluids.

A method is described for the determination of thioridazine and its major metabolites in postmortem tissues and fluids by enzymic digestion, followed by a micro-extraction technique using ethyl acetate. Quantitation was performed by using a cyano-bonded silica (mu Bondapak-CN) column packing and a mobile phase consisting of n-octylamine, methanol, and water.