Kidney international · 2005
Toll-like receptor-4: renal cells and bone marrow cells signal for neutrophil recruitment during pyelonephritis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Patole Prashant S, Schubert Sören, Hildinger Kirsten, Khandoga Sascha, Khandoga Andrej, Segerer Stephan, Henger Anna, Kretzler Matthias, Werner Melanie, Krombach Fritz, Schlöndorff Detlef, Anders Hans-Joachim
Аннотация
BACKGROUND: The molecular mechanisms of pathogen recognition that initiate infective pyelonephritis are poorly understood. Toll-like receptor-4 (TLR4) mutant mice infected with uropathogenic Escherichia coli lack renal CXCL2 mRNA expression, subsequent neutrophil recruitment, and renal abscess formation.
METHODS: We used a bone marrow transplant approach in order to investigate the contribution of TLR4 in intrinsic renal cells or bone-marrow-derived immune cells to neutrophil recruitment during infective pyelonephritis.
RESULTS: Both chimera either expressing mutant tlr4 in intrinsic renal cells and wild-type tlr4 in bone marrow-derived cells or vice versa showed an impaired response to uropathogenic E. coli infection in terms of leukocyturia and renal abscess formation when compared to tlr4 wild-type mice with congenic bone marrow transplants.
CONCLUSION: These data suggest that TLR4 is required on both intrinsic renal cells (e.g., tubular epithelial cells) and bone marrow-derived immune cells for the control of ascending uropathogenic E. coli infection by initiating chemokine-driven renal neutrophil recruitment.
METHODS: We used a bone marrow transplant approach in order to investigate the contribution of TLR4 in intrinsic renal cells or bone-marrow-derived immune cells to neutrophil recruitment during infective pyelonephritis.
RESULTS: Both chimera either expressing mutant tlr4 in intrinsic renal cells and wild-type tlr4 in bone marrow-derived cells or vice versa showed an impaired response to uropathogenic E. coli infection in terms of leukocyturia and renal abscess formation when compared to tlr4 wild-type mice with congenic bone marrow transplants.
CONCLUSION: These data suggest that TLR4 is required on both intrinsic renal cells (e.g., tubular epithelial cells) and bone marrow-derived immune cells for the control of ascending uropathogenic E. coli infection by initiating chemokine-driven renal neutrophil recruitment.
Предметные рубрики MeSH
AnimalsBone Marrow CellsBone Marrow TransplantationCells, CulturedChemokine CXCL2ChemokinesChemotaxis, LeukocyteEpithelial CellsEscherichia coli InfectionsKidney TubulesLipopolysaccharidesMiceMice, Inbred C3HMice, Mutant StrainsMutant Chimeric ProteinsNeutrophilsPyelonephritisRNA, MessengerSignal TransductionToll-Like Receptor 4