Fertility and sterility · 1998
Microsatellite mutations in spontaneously aborted embryos.
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Spandidos D A, Koumantakis E, Sifakis S, Sourvinos G
Аннотация
OBJECTIVE: To evaluate the incidence of microsatellite instability in spontaneously aborted embryos.
DESIGN: Retrospective study.
SETTING: Laboratory of Clinical Virology and Department of Obstetrics and Gynecology, University Hospital, Medical School, University of Crete.
PATIENT(S): Thirty-five women in whom spontaneous abortions occurred between the 6th and 20th weeks of pregnancy.
INTERVENTION(S): Thirty-five aborted embryonic tissues were analyzed with seven microsatellite markers, and their haplotypes were compared with the corresponding pattern of their parents.
MAIN OUTCOME MEASURE(S): Microsatellite DNA.
RESULT(S): Microsatellite instability was observed in 8 of 35 cases (23%). In 7 of 8 positive cases, microsatellite instability was restricted to one of the seven microsatellite markers, whereas in one case, three microsatellite markers were affected by instability. A statistically significant association was found between microsatellite instability and a previous normal childbirth.
CONCLUSION(S): Genetic instability is a detectable phenomenon in spontaneous abortions, representing a significant increase in the mutational rate of the embryo and providing evidence for a mechanism associated with the phenomenon of spontaneous abortion. We conclude that this elevated mutational rate affects active genomic sequences that play a critical role in the viability of the embryo, leading to cell death and abortion.
DESIGN: Retrospective study.
SETTING: Laboratory of Clinical Virology and Department of Obstetrics and Gynecology, University Hospital, Medical School, University of Crete.
PATIENT(S): Thirty-five women in whom spontaneous abortions occurred between the 6th and 20th weeks of pregnancy.
INTERVENTION(S): Thirty-five aborted embryonic tissues were analyzed with seven microsatellite markers, and their haplotypes were compared with the corresponding pattern of their parents.
MAIN OUTCOME MEASURE(S): Microsatellite DNA.
RESULT(S): Microsatellite instability was observed in 8 of 35 cases (23%). In 7 of 8 positive cases, microsatellite instability was restricted to one of the seven microsatellite markers, whereas in one case, three microsatellite markers were affected by instability. A statistically significant association was found between microsatellite instability and a previous normal childbirth.
CONCLUSION(S): Genetic instability is a detectable phenomenon in spontaneous abortions, representing a significant increase in the mutational rate of the embryo and providing evidence for a mechanism associated with the phenomenon of spontaneous abortion. We conclude that this elevated mutational rate affects active genomic sequences that play a critical role in the viability of the embryo, leading to cell death and abortion.