Wei sheng wu xue bao = Acta microbiologica Sinica · 2015
[Construction of genetically engineered strain producing 5- oxomilbemycin by knocking out milF in Steptomyces hygroscopicus HS023].
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Huang Jun, Lin Jiatan, Zhou Min, Bai Hua
Аннотация
OBJECTIVE: To construct a 5-oxomilbemycin producing strain by knocking out milF gene in Streptomyces hygroscopicus HS023.
METHODS: Plasmid pMSST-ΔmilF was constructed and introduced into milbemycin industrial strain Streptomyces hygroscopicus HS023, and milF mutant F2-18 was selected by PCR amplification.
RESULTS: Fermentation experiments showed that no milbemycins was produced in F2-18, but 5-ketomilbemycin, the intermediate of milbemycin, was obviously accumulated, and the fermentation titer was enhanced.
CONCLUSION: Genetically engineered strain can simplify the synthesis of milbemycin oxime and lepimectin chemical from milbemycin.
METHODS: Plasmid pMSST-ΔmilF was constructed and introduced into milbemycin industrial strain Streptomyces hygroscopicus HS023, and milF mutant F2-18 was selected by PCR amplification.
RESULTS: Fermentation experiments showed that no milbemycins was produced in F2-18, but 5-ketomilbemycin, the intermediate of milbemycin, was obviously accumulated, and the fermentation titer was enhanced.
CONCLUSION: Genetically engineered strain can simplify the synthesis of milbemycin oxime and lepimectin chemical from milbemycin.