[Construction of genetically engineered strain producing 5- oxomilbemycin by knocking out milF in Steptomyces hygroscopicus HS023].

OBJECTIVE

To construct a 5-oxomilbemycin producing strain by knocking out milF gene in Streptomyces hygroscopicus HS023.

METHODS

Plasmid pMSST-ΔmilF was constructed and introduced into milbemycin industrial strain Streptomyces hygroscopicus HS023, and milF mutant F2-18 was selected by PCR amplification.

RESULTS

Fermentation experiments showed that no milbemycins was produced in F2-18, but 5-ketomilbemycin, the intermediate of milbemycin, was obviously accumulated, and the fermentation titer was enhanced.

CONCLUSION

Genetically engineered strain can simplify the synthesis of milbemycin oxime and lepimectin chemical from milbemycin.