Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology · 2005
[Construction of infectious Japanese encephalitis virus clone based on the cDNA template of the attenuated live vaccine production strain SA14-14-2].
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zeng Ming, Jia Li-li, Yu Yong-xin, Dong Guan-mu, Liu Wen-xue, Wang Zhi-wei, Li De-fu
Аннотация
OBJECTIVE: To construct infectious Japanese encephalitis virus (JEV) based on the in vitro-ligated cDNA template of the vaccine strain SA14-14-2, and identify the virus.
METHODS: Full-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified.
RESULTS: The infective clone of JEV was constructed, the virulence was weaker than the wild virus.
CONCLUSION: It was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.
METHODS: Full-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified.
RESULTS: The infective clone of JEV was constructed, the virulence was weaker than the wild virus.
CONCLUSION: It was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.