On-line determination of trace sulfur dioxide in air by integrated microchip coupled with fluorescence detection.

A microchip-based method was developed for on-line determination of trace sulfur dioxide (SO(2)) in air. Gaseous SO(2), which diffused through the porous glass materials on the microchip, was absorbed into an absorption solution of triethanolamine (TEA) as sulfite ions and reacted with N-(9-acridinyl)maleimide (NAM), which was used as a fluorescent reagent. The fluorescence of NAM-sulfite in micro-fluidic channel was detected. The calibration curve of sulfite ions in the range of 1.5-30mumol/L (SO(2) 3-60ppbv) showed a linear relation R(2)=0.995, and the relative standard deviation (R.S.D.) was 1.9% for 10mumol/L sulfite ions in five measurements. The entire measurement procedure was achieved by the integrated microchip, and the consumption of reagents was drastically reduced. It was satisfactory to apply this method to determine on-line the SO(2) level in the air.