Strong and robust cellulose-based enzymatic membrane with gradient porous structure in dynamically catalytic removal of sulfonamides antibiotics.

Enzyme membrane systems (EMS) have generated considerable interest because of their advantages of accelerating reactions, eliminating product inhibition, and enhancing conversion rates. However, there are deficiencies in the efficient fabrication of affinity carrier membranes and dynamic catalytic separation properties. Herein, a strong and highly flexible spunlaced viscose/bacterial cellulose (BC) composite membrane in situ embedded with graphene oxide (GO) was developed by combining a scalable bio-synthesis method with atom transfer radical polymerization technology. Notably, the layer-by-layer growth of BC on composite film and the addition of GO resulted in an entangled network with strong hydrogen bonding, endowing the resulting membrane with superior mechanical properties and flexibility, while facilitating a gradient structure and porous transport channels. Subsequently, a novel and highly efficient EMS was constructed by using abundant molecular brushes on composite membrane as immobilized enzyme carrier. The resulting EMS exhibited a high throughput (2.17 L/min*m2) and an interception rate (98.64%) in dynamic catalytic sulfonamide antibiotic wastewater activated with syringaldehyde mediator. Meanwhile, the removal rates of sulphapyridine and sulfamethazine were 97.20% and 94.78% under 0.14 MPa and 15 min, respectively. This efficient and scalable manufacturing strategy is of great significance and may pave a novel pathway for antibiotics wastewater treatment and recycling.