Chinese medical journal · 1997
Pregnancies from subzonal insemination of unfertilized oocytes after conventional IVF.
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Loong E P, Chiu T T, Haines C J
Аннотация
OBJECTIVE: To evaluate the efficacy of subzonal insemination (SUZI) as applied to unfertilized oocytes after initial insemination.
METHODS: Unfertilized oocytes at metaphase II obtained after conventional IVF from 14 patients were selected for reinsemination by SUZI. The fertilization rate and pregnancy outcome were assessed.
RESULTS: Of the 57 unfertilized oocytes at metaphase II, 10 (17.5%) became fertilized after SUZI. Eight of the 10 (80%) oocytes showed normal fertilization with two pronuclei while the other two (20%) oocytes were polyspermic. Five (35.7%) of the 14 patients had embryo transfer resulting in two (14.3%) pregnancies. One patient had a spontaneous abortion and the other had a live birth at term.
CONCLUSION: Reinsemination of unfertilized oocytes by SUZI could be of value when fertilization fails after initial insemination.
METHODS: Unfertilized oocytes at metaphase II obtained after conventional IVF from 14 patients were selected for reinsemination by SUZI. The fertilization rate and pregnancy outcome were assessed.
RESULTS: Of the 57 unfertilized oocytes at metaphase II, 10 (17.5%) became fertilized after SUZI. Eight of the 10 (80%) oocytes showed normal fertilization with two pronuclei while the other two (20%) oocytes were polyspermic. Five (35.7%) of the 14 patients had embryo transfer resulting in two (14.3%) pregnancies. One patient had a spontaneous abortion and the other had a live birth at term.
CONCLUSION: Reinsemination of unfertilized oocytes by SUZI could be of value when fertilization fails after initial insemination.