Microbial drug resistance (Larchmont, N.Y.) · 2014
A novel Tn3-like composite transposon harboring blaVIM-1 in Klebsiella pneumoniae spp. pneumoniae isolated from river water.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Zurfluh Katrin, Power Karen A, Klumpp Jochen, Wang Juan, Fanning Séamus, Stephan Roger
Аннотация
OBJECTIVES: We present a new plasmid (pOW16C2) with a novel Tn3-like transposon harboring blaVIM-1 from a Klebsiella pneumoniae strain isolated from river water in Switzerland.
METHODS: Complete nucleotide sequence of pOW16C2 was obtained using a Pacific Biosciences SMRT sequencing approach and coding sequences were predicted.
RESULTS: The 59,228 bp sequence included a typical IncN-like backbone and a mosaic structure with blaVIM-1, aacA4, aphA15, aadA1, catB2, qnrS1, sul1, and dfrA14 conferring resistance to carbapenems and other β-lactam antibiotics, aminoglycosides, chloramphenicol, quinolones, sulfonamides, and trimethoprim, respectively. Most of these resistance genes were inserted in a class 1 integron that was embedded in a novel Tn3-like composite transposon.
CONCLUSIONS: IncN plasmids carrying carbapenemases are frequently isolated from K. pneumoniae strains in clinical settings. The dissemination of K. pneumoniae harboring blaVIM-1 in surface water is a cause for increased concern to public health.
METHODS: Complete nucleotide sequence of pOW16C2 was obtained using a Pacific Biosciences SMRT sequencing approach and coding sequences were predicted.
RESULTS: The 59,228 bp sequence included a typical IncN-like backbone and a mosaic structure with blaVIM-1, aacA4, aphA15, aadA1, catB2, qnrS1, sul1, and dfrA14 conferring resistance to carbapenems and other β-lactam antibiotics, aminoglycosides, chloramphenicol, quinolones, sulfonamides, and trimethoprim, respectively. Most of these resistance genes were inserted in a class 1 integron that was embedded in a novel Tn3-like composite transposon.
CONCLUSIONS: IncN plasmids carrying carbapenemases are frequently isolated from K. pneumoniae strains in clinical settings. The dissemination of K. pneumoniae harboring blaVIM-1 in surface water is a cause for increased concern to public health.
Предметные рубрики MeSH
Anti-Bacterial AgentsBacterial Typing TechniquesBase SequenceDNA Transposable ElementsDNA, BacterialDisk Diffusion Antimicrobial TestsDrug Resistance, Multiple, BacterialGenes, BacterialKlebsiella pneumoniaeMolecular Sequence DataMultilocus Sequence TypingPlasmidsRiversSequence Homology, Nucleic AcidSwitzerlandWater Microbiologybeta-Lactamases