Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2000
[The influence of epidermal growth factor, neurotensin on cytosolic calcium and membrane fluidity in carbon tetrachloride-injured primary cultured hepatocytes].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zhang L, Cai L, Zheng Y, Wei Y, Pan X
Аннотация
OBJECTIVE: To study the influence of epidermal growth factor (EGF), neurotensin (NT) on cytosolic calcium and membrane fluidity in carbon tetrachloride-injured primary cultured hepatocytes.
METHODS: A micro-model of carbon tetrachloride -induced primary cultured hepatocytes injury was established. EGF and NT were added to the cells 1 h ahead of carbon tetrachloride given. Twenty-four hours later, cytosolic calcium and membrane fluidity in hepatocytes were detected with fluorescence probe of Fura-2/AM and 1, 6-dihenyl-1, 3, 5-hexatriene (DPH).
RESULTS: EGF and NT could significantly antagonize the increase of cytosolic Ca(2+) and decrease of membrane fluidity in carbon tetrachloride-injured hepatocytes.
CONCLUSION: EGF and NT have a function of maintaining membrane fluidity and steady-status of cytosolic calcium of hepatocytes.
METHODS: A micro-model of carbon tetrachloride -induced primary cultured hepatocytes injury was established. EGF and NT were added to the cells 1 h ahead of carbon tetrachloride given. Twenty-four hours later, cytosolic calcium and membrane fluidity in hepatocytes were detected with fluorescence probe of Fura-2/AM and 1, 6-dihenyl-1, 3, 5-hexatriene (DPH).
RESULTS: EGF and NT could significantly antagonize the increase of cytosolic Ca(2+) and decrease of membrane fluidity in carbon tetrachloride-injured hepatocytes.
CONCLUSION: EGF and NT have a function of maintaining membrane fluidity and steady-status of cytosolic calcium of hepatocytes.