Gene · 2026
PBX1-mediated transcription of AP1M2 promotes triple-negative breast cancer malignant progression and docetaxel resistance.
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Cui Ruijie, Chen Hongli, Zhao Xin, Xu Sheng, Fang Bing
Аннотация
BACKGROUND: Adaptor-related protein complex 1 subunit mu 2 (AP1M2) has been has been shown to be overexpressed in breast cancer tissues. However, whether AP1M2 regulates triple-negative breast cancer (TNBC) progression and chemoresistance is unknown.
METHODS: The expression of AP1M2 and pre-B-cell leukaemia homeobox 1 (PBX1) was analyzed by qRT-PCR or western blot. Cell proliferation, migration, invasion, docetaxel resistance, and apoptosis were analyzed using CCK8 assay, colony formation assay, EdU assay, transwell assay, wound healing assay and flow cytometry. Dual-luciferase reporter assay was used to assess the interaction between AP1M2 and PBX1.
RESULTS: AP1M2 had increased expression in TNBC cells, and its knockdown suppressed TNBC cell proliferation, migration, invasion, and enhanced docetaxel sensitivity. In terms of mechanism, PBX1 bound to AP1M2 promoter region to enhance its transcription. The rescue experiments revealed that overexpression of AP1M2 could abolish the suppressive effect of PBX1 knockdown on TNBC cell proliferation, migration, invasion, and docetaxel resistance.
CONCLUSION: PBX1-mediated AP1M2 facilitates cell proliferation, migration, invasion, and docetaxel resistance to accelerate TNBC malignant behavior, providing a novel target for TNBC treatment.
METHODS: The expression of AP1M2 and pre-B-cell leukaemia homeobox 1 (PBX1) was analyzed by qRT-PCR or western blot. Cell proliferation, migration, invasion, docetaxel resistance, and apoptosis were analyzed using CCK8 assay, colony formation assay, EdU assay, transwell assay, wound healing assay and flow cytometry. Dual-luciferase reporter assay was used to assess the interaction between AP1M2 and PBX1.
RESULTS: AP1M2 had increased expression in TNBC cells, and its knockdown suppressed TNBC cell proliferation, migration, invasion, and enhanced docetaxel sensitivity. In terms of mechanism, PBX1 bound to AP1M2 promoter region to enhance its transcription. The rescue experiments revealed that overexpression of AP1M2 could abolish the suppressive effect of PBX1 knockdown on TNBC cell proliferation, migration, invasion, and docetaxel resistance.
CONCLUSION: PBX1-mediated AP1M2 facilitates cell proliferation, migration, invasion, and docetaxel resistance to accelerate TNBC malignant behavior, providing a novel target for TNBC treatment.
Предметные рубрики MeSH
HumansDocetaxelTriple Negative Breast NeoplasmsPre-B-Cell Leukemia Transcription Factor 1Drug Resistance, NeoplasmFemaleCell Line, TumorCell ProliferationCell MovementGene Expression Regulation, NeoplasticApoptosisAntineoplastic AgentsDisease ProgressionTranscription, GeneticPromoter Regions, Genetic