Biochemical and biophysical research communications · 2004
Potentiality of DNA-dependent protein kinase to phosphorylate Ser46 of human p53.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Komiyama Shingo, Taniguchi Sachiko, Matsumoto Yoshihisa, Tsunoda Eri, Ohto Takayo, Suzuki Yuka, Yin Hong-Lan, Tomita Masanori, Enomoto Atsushi, Morita Akinori, Suzuki Takahiko, Ohtomo Kuni, Hosoi Yoshio, Suzuki Norio
Аннотация
DNA damage induces accumulation and activation of p53 via various posttranslational modifications. Among them, several lines of evidence indicated the phosphorylation of Ser46 as an important mediator of DNA damage-induced apoptosis but the responsible kinase remains to be clarified, especially in the case of ionizing radiation (IR). Here we showed that DNA-dependent protein kinase (DNA-PK) could phosphorylate Ser46 of p53 in addition to reported phosphorylation sites Ser15 and Ser37. However, IR-induced phosphorylation of Ser46 was seen even in M059J, a human glioma cell line lacking DNA-PKcs, and it was, at most, only slightly less than in control M059K. On the other hand, a related kinase ataxia-telangiectasia mutated (ATM), which was shown to be essential for IR-induced phosphorylation of Ser46, could poorly phosphorylate Ser46 by itself. These results collectively suggested two pathways for IR-induced phosphorylation of Ser46, i.e., direct phosphorylation by DNA-PK and indirect phosphorylation via ATM.