Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2007
[Mechanism of the effect of LPSp on the production of anti-HBs in mice].
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Wang Jian, Leng Jing, Wang Qi-hui
Аннотация
AIM: To investigate the mechanism of the effect of avirulent Pantoea agglomerans lipopolysaccharide (LPSp) as the adjuvant of HBsAg on the production of anti-HBs in mice.
METHODS: The peritoneal macrophages of BALB/c mice were pulsed in vitro by HBsAg+LPSp, HBsAg, LPSp, and NS respectively. The levels of anti-HBs IgG in serum were determined by ELISA. The variation of phagocytosis of the macrophages was detected. The culture supernatants were harvested and assayed for TNF-alpha and NO. HBsAg-pulsed peritoneal macrophages in the presence or absence of LPSp were injected in the hind footpads of syngeneic mice. Lymph node cells were harvested and cultured by HBsAg. IL-4 and IFN-gamma in the supernatants were quantitated by ELISA.
RESULTS: The titer of anti-HBs IgG from the mice immunized with HBsAg-pulsed peritoneal macrophages treated with LPSp was higher than that of the mice immunized in the absence of LPSp. The phagocytosis rate of the LPSp-treated peritoneal macrophages was higher than that of the untreated macrophages. The level of TNF-alpha and NO of the LPSp-treated peritoneal macrophages was higher than that of the untreated macrophages. HBsAg-pulsed peritoneal cells in the presence of LPSp can induced higher production of IL-4 than those in the absence of LPSp.
CONCLUSION: LPSp can act as the adjuvant of HBsAg and effectively increase the anti-HBS in the mice immunized by HBsAg. LPSp can activate macrophages and stimulate HBsAg-pulsed macrophages to induce the production of IL-4 of lymph node cells.
METHODS: The peritoneal macrophages of BALB/c mice were pulsed in vitro by HBsAg+LPSp, HBsAg, LPSp, and NS respectively. The levels of anti-HBs IgG in serum were determined by ELISA. The variation of phagocytosis of the macrophages was detected. The culture supernatants were harvested and assayed for TNF-alpha and NO. HBsAg-pulsed peritoneal macrophages in the presence or absence of LPSp were injected in the hind footpads of syngeneic mice. Lymph node cells were harvested and cultured by HBsAg. IL-4 and IFN-gamma in the supernatants were quantitated by ELISA.
RESULTS: The titer of anti-HBs IgG from the mice immunized with HBsAg-pulsed peritoneal macrophages treated with LPSp was higher than that of the mice immunized in the absence of LPSp. The phagocytosis rate of the LPSp-treated peritoneal macrophages was higher than that of the untreated macrophages. The level of TNF-alpha and NO of the LPSp-treated peritoneal macrophages was higher than that of the untreated macrophages. HBsAg-pulsed peritoneal cells in the presence of LPSp can induced higher production of IL-4 than those in the absence of LPSp.
CONCLUSION: LPSp can act as the adjuvant of HBsAg and effectively increase the anti-HBS in the mice immunized by HBsAg. LPSp can activate macrophages and stimulate HBsAg-pulsed macrophages to induce the production of IL-4 of lymph node cells.