Cardioprotective potential of indol-3-carbinol against high salt induced myocardial stress and hypertrophy in Sprague dawley rats besides molecular docking on muscarinic receptor-2.

The vegetative chemical constituent, indol-3-carbinol (I-3-C) studied for its cardioprotective potential in male Sprague dawley rats. The I-3-C at 20 mg/Kg b.w, p.o significantly (p < 0.001) attenuated the high salt induced hypertrophy and produced antihypertensive effect (p < 0.001) as similar to losartan. Further, it significantly reduced the levels of C-reactive protein (p < 0.05), creatinine kinases isoenzyme (p < 0.01), serum lactate dehydrogenase (p < 0.05), myeloperoxidase (p < 0.01) and hydroxyproline (p < 0.01), subsequently increased the nitric oxide level (p < 0.05). The carotid ligation for vascular reactivity against vasopressors revealed a lesser magnitude of change (p < 0.05) in invasive blood pressure for I-3-C, compared to high salt treated animals (p < 0.001). In histology of heart tissue also supported the cardioprotective effect of I-3-C. In silico molecular docking of I-3-C on muscarinic receptor-2 showed the amino acid interaction as similar to acetylcholine.